To analyze the KRAS WT and mutant interactomes in different growth media (“culture contexts”) that mimic conditions relevant in the colon and CRC, Caco-2 cells were grown 4 h after transfection for 24 h in minimal medium (DMEM with 2 mM l-glutamine) supplemented with either IL-6, TNF-α, PGE2, EGF, or the HIF-hydroxylase inhibitor DMOG at different concentrations (20 and 200 ng/ml) before the AP-MS experiment was conducted. Here, KRAS is linked to colorectal carcinoma.