Additionally, bosentan competitively bound to ET-1 receptors and inhibited the miR-27a/PPARγ/ET-1 signalling pathway, thereby delaying PASMC proliferation and affecting PAH development [41].Our study also suggested that the PPAR signalling pathway was related to PAH regulation and the enrichment of upregulated mRNAs in this pathway was relatively high. This evidence concerns the gene EDN1 and pulmonary arterial hypertension.