DIO1 and metabolic dysfunction-associated steatotic liver disease: Although in non-alcoholic steatohepatitis (WDF 16 and 24 weeks) there is increased expression of pro-inflammatory cytokines (IL6, IL1-β, TNFα), Dio1 mRNA does not decrease below the level of mice fed a normal chow diet after 24 weeks of diet (31, 46) This shows that in this obesogenic NAFLD model, the presence of inflammatory cytokines does not directly imply decreased deiodinase activity measured in whole liver; however, the diet-induced increase is dampened.