VSIR and acute myeloid leukemia: Additionally, VISTA is expressed on CD11b+ myeloid cells in the TME of melanoma, oral squamous cell carcinoma and in AML, where high expression of VISTA was detected on monocytes 46% (gated on CD45+CD11b+CD14hi/lo) and myeloid leukemia blasts 23% (gated by CD45int, CD45 vs. side scatter) and VISTA+ MDSCs 56% (42–44).