All these results indicated that CDKN2A-hsa-miR-125b-5p, GLS-hsa-miR-125b-5p, GLS-hsa-miR-101–3, GLS-hsa-miR-23c and PDHA1-hsa-miR-125b-5p might represent key pathways that mediated the occurrence and development of HCC and were related to the prognosis of patients. Here, GLS is linked to hepatocellular carcinoma.