The KEGG analysis showed that the DEGs were mainly enriched in focal adhesion, extracellular matrix-receptor interaction, PI3K-Akt signaling pathway, TGF-β signaling pathway, HCC, cell cycle, NOD-like receptor signaling pathway, PD-L1 expression and the PD-1 checkpoint pathway in cancer (Figure 3D). This evidence concerns the gene PDCD1 and hepatocellular carcinoma.