In the present study, we explored the diagnosis of AFP-negative HCC using conventional laboratory examination data in combination with several biomarkers, focusing on γ-glutamine aminotransferase-to-PA ratio (GPR) and γ-glutamine aminotransferase-to-glutathione aminotransferase (GAR), and clinicopathological indicators to assess their feasibility as predictive markers for patients with AFP-negative HCC. Here, AFP is linked to hepatocellular carcinoma.