In addition, downregulation of actin filament-associated protein 1-antisense RNA 1 (Actin filament-associated protein 1-antisense RNA1, AFAP1-AS1) and cancer-associated region long non-coding RNA (CARLo-5) can cause cells to undergo S-phase arrest, and the expression of S-phase-related proteins CDK2 and Cyclin A2 will be down-regulated to varying degrees, thereby inhibiting the proliferation of HeLa cells. The gene discussed is AFAP1; the disease is cancer.