Our study is the first to analyze CAT as a metabolite of gut microbiota in mice with osteoarthritis, and in addition, CAT inhibited HIF‐1a expression and reduced ferroptosis‐dependent osteoarthritis progression by activating SLC2A1, which provides an important strategy for CAT as a potential drug for the treatment of osteoarthritis. The gene discussed is CAT; the disease is osteoarthritis.