Our findings that EGFR and HER3 are expressed in B16 melanoma cells (Supplemental Figure 5, A and B), and that rCL-11 in cell cultures rapidly results in phosphorylation of EGFR and HER3 — together with the fact that EGFR and HER3 are highly glycosylated proteins containing multiple N-glycans (49), which could be potential binding sites for CL-11 — provide strong evidence to support the notion that CL-11 could mediate melanoma cell proliferation through interaction with EGFR and HER3. Here, EGFR is linked to melanoma.