However, of the 8 cell lines tested (4 healthy and 4 FSHD), we could only detect DUX4c in one FSHD cell line, in which we observed a weak band at the expected 47-kDa size in the total and nuclear extracts, but not in the cytoplasmic extract. The gene discussed is DUX4L9; the disease is facioscapulohumeral muscular dystrophy.