To investigate whether miR-146b-5p/SEMA3G modulates ccRCC cell phenotypes through the Notch and TGF-β signaling, we constructed a SEMA3G overexpression cell line (miR-NC + oe-SEMA3G) and a simultaneous overexpression cell line of miR-146b-5p and SEMA3G (miR-mimic + oe-SEMA3G) using Caki-2 cell line. The gene discussed is TGFB1; the disease is nonpapillary renal cell carcinoma.