EMT process and the activities of Notch and TGF-β signaling pathways were inhibited in ccRCC cells during SEMA3G overexpression, but after simultaneous increasing miR-146b-5p and SEMA3G, EMT process and activities of the Notch and TGFβ signaling pathways in ccRCC cells were notably increased compared with those when SEMA3G was overexpressed alone. All in all, the results above demonstrated that miR-146b-5p facilitated migration, invasion, and EMT of tumor cells by down-regulating SEMA3G via the Notch and TGF-β signaling pathways. This evidence concerns the gene TGFB1 and nonpapillary renal cell carcinoma.