TP53 and cancer: In contrast, T.S 100% EE and T.S 50% EE treated HepG2 and Caco-2 cancer cells significantly have the lowest percentage of apoptotic cells and failed in upregulating p53 gene expression and downregulation of BCL2, Cyclin D, MMP9 and VEGF signaling pathway. p53 inactivation is linked to about 50% of cancer cases.