Since the minimal detectable variation in the p16INK4A‐positive ratio (i.e., highest resolution) using FCM reached 0.1% at the reference cut‐off of 10% (i.e., 10 cells per 10000‐cell sample; Figure 1D and Table S1), which satisfied the requirement for the establishment of an effective cervical cancer screening program, we then applied the reference cut‐off of 10% as a routine FCM setting for testing clinical samples in the next tasks in our study. This evidence concerns the gene CDKN2A and cervical carcinoma.