NECTIN1 and infection: We therefore transduced B16 melanoma cells with a lentivirus encoding human nectin-1,45 a known HSV-1 entry receptor.46 Whereas B16WT cells could not be infected with d106S-GFP, B16Nectin1 cells were readily infected with the virus; titration of d106S-GFP virus on B16Nectin1 alongside a panel of five human melanoma cell lines showed comparable levels of infection (Figure S1B).