We examined epithelial subtype markers and found that small intestinal tissue from neonates with NEC demonstrated decreased expression of stem cell (leucine-rich repeat–containing G protein–coupled receptor 5, LGR5), Paneth cell (lysozyme, LYZ), and goblet cell markers (MUC2) compared with control tissue (Figure 2A). This evidence concerns the gene LGR5 and necrotizing enterocolitis.