That depletion of Class I ARFs induced invasive activity was somewhat unexpected, as numerous studies, particularly in breast cancer cells, report a pro-invasive and pro-tumorigenic function of ARF1 (Boulay et al., 2008; Boulay et al., 2011; Haines et al., 2015; Lewis-Saravalli et al., 2013; Schlienger et al., 2014; Schlienger et al., 2016; Schlienger et al., 2015; Wang et al., 2020; Xie et al., 2016). Here, ARF1 is linked to breast cancer.