An adenovirus armed with CD40L and 4‐1BBL; activates CD40 and 4‐1BB pathways; Decrease the tumor growth factors (including Spp‐1, Gal‐3, HGF, TGFβ, and collagen type I); Increase cytokines and chemokines, and such DCs potently expanded both antigen‐specific T cells and NK cells. This evidence concerns the gene LGALS3 and neoplasm.