In this study, we take advantage of a classic genetic modifier screen approach to identify bona fide regulators of a 4-hit model targeting Drosophila orthologs of KRAS, TP53, PTEN, and APC, 4 genes recurrently mutated in colon tumors, as well as KRAS alone, which represents one of the most commonly observed pathway alterations in human tumors (Cancer Genome Atlas Network 2012). This evidence concerns the gene APC and colonic neoplasm.