Our present study further showed that GABAA receptors antagonist bicuculline blocked the inhibitory effects of TGR5 or FXR agonists on neuropathic pain behaviors and on the activation of glial cells, which has been reported to be critical to the development and maintenance of neuropathic pain by initiating the neuroinflammatory response after peripheral nerve injury. This evidence concerns the gene GPBAR1 and peripheral nerve injury.