CD163 and neoplasm: To investigate whether the tumor-associated macrophages which were positivelystained for CD68 in the mIHC method were likely to be of an M2 phenotype (i.e.,stained for both CD68 and CD163), we used a standard IHC method to semi-quantifythe amount of CD163+ cells and visually inspected the serially CD68and CD163 stained sections.