The purpose of our study was to optimize an mIHC method for PCa biopsies and use itto study infiltration of T-bet+ Th1-cells, CD8+ cytotoxicT-cells, CD20+ B-cells, macrophages, and FOXP3+ Tregs in PCatissue before and after castration with 8 weeks ADT, RT to a total dose of 10 Gy, ora combination of both treatments. This evidence concerns the gene CD8A and posterior cortical atrophy.