Finally, through combination of clinical data from the GEO database with qRT‒PCR analysis results, we verified that integrin subunit α-4 (ITGA4), inositol 1,4,5-trisphosphate receptor type 2 (ITPR2), adhesion G protein-coupled receptor E2 (ADGRE2), ankyrin repeat domain 28 (ANKRD28), lysine demethylase 5B (KDM5B) and cyclin-dependent kinase 6 (CDK6) were significantly upregulated in NK-AML and that ITGA4 and ITPR2 may be biomarkers for predicting NK-AML prognosis. Here, ANKRD28 is linked to acute myeloid leukemia.