Moreover, upregulation of ATP6AP2, TMEM97, CDCA2, and CCND3 led to a significant increase in the proliferation in BC cells (Fig. 4H and Additional file 3: Fig S3C), overexpression of TMEM97, CCND3, and AACS resulted in significantly increased migration and invasion abilities of BC cells (Fig. 4I and Additional file 3: Fig S3D). This evidence concerns the gene AACS and breast cancer.