Random survival forest analysis of DEGs from the TCGA-READ cohort demonstrated eight genes accorded with the threshold, including KCNMA1, TMC1, ALG10, HGD, HOXD3, CDKN2D, PLAGL2 and ZNF337. Finally, PLAGL2, ZNF337 and ALG10 were significantly associated with the survival of READ patients and identified as hub genes of current study. This evidence concerns the gene CDKN2D and reading.