The results showed the expressions of multiple disease-related genes were significant different between control group and READ group, which included genes of TP53, MET, MSH2, PTEN, PIK3CA, MSH6, KRAS, EGFR, CTNNB1, CDKN2A, RET, PMS2, HRAS, BRCA1 and APC (Fig. 4A). The gene discussed is MSH2; the disease is reading.