Comparatively, of the enriched KEGG pathways involved by DEGs from aging RGCs in USCs medium, the top 10 included Cytokine-cytokine receptor interaction, JAK-STAT signaling pathway, Circadian entrainment, Proximal tubule bicarbonate reclamation, MicroRNAs in cancer Viral, protein interaction with cytokine and cytokine receptor Chemokine signaling pathway, Mineral absorption, Protein digestion and absorption, Apelin signaling pathway TNF signaling pathway, Aldosterone-regulated sodium reabsorption. The gene discussed is APLN; the disease is cancer.