Notably, the siRNA-mediated knockdown of L2HGDH or OGDH, but not D2HG, upregulated L2HG in CRC cells (Fig. 5B; Supplementary Fig. S5A–D), with their combined knockdown further enhancing L2HG induction (Fig. 5B). This evidence concerns the gene OGDH and colorectal carcinoma.