Therefore, we performed hydrodynamic tail-vein injection (HDTVi) of px330-sg-p53 and CMV-SB13 combined with CTNNB1-N90 into 6-week-old C57BL/6J mice to establish CTNNB1N90;Trp53KO HCC murine models to mimic the HCC genetic background (Fig. 2a)15,16. The gene discussed is TP53; the disease is hepatocellular carcinoma.