Using CD36, CD146, FAP and MHCII antibodies, CAFs were segregated into four populations: (1) CD36-positive, (2) MHCII-positive, (3) FAP-positive, and (4) CD146-positive cells, presumably corresponding to CD36+ CAFs, apCAFs, mCAFs and vCAFs, respectively (Fig. 4a), and these results were also validated in HCC tissues using mIF assays (Supplementary Fig. S8a). This evidence concerns the gene FAP and hepatocellular carcinoma.