Of particular interest, splenocytes from early immunized RIP-B7.1 mice were also found to upregulate MIF, evidencing that EAD development is associated with a splenic inflammatory process, a premise consistent with previous reports indicating a role for MIF expression in the development of T1DM and as critical regulator of innate immunity (Calandra and Roger 2003; Stosic-Grujicic et al. 2008; Sanchez-Zamora et al. 2016). This evidence concerns the gene CD80 and type 1 diabetes mellitus.