EGFR and neoplasm: Fluorescence image normalization reduced variations in inter-patient signals and amplified differences in normal and tumor fluorescence; however, inhomogeneous overexpression of EGFR in tumor and high endogenous expression of EGFR in normal tissues still have confounding effects on fluorescence signals, which is evident in the overlapping regions of intensity histograms from ground truth tumor and normal tissue ROIs displayed in Supplementary Figure S7.