Our scRNA-seq studies from tumor-infiltrating T lymphocytes revealed that the Emut Vax not only promotes the generation of Th1 helper CD4+ cells with enhanced function but also induces Th9 and Th17 cells, further cytokine analysis also confirmed the upregulated IL1, IL-4, IL9 and IL17 etc. after Emut peptide restimulation. This evidence concerns the gene CD4 and neoplasm.