IRS1 and metabolic dysfunction-associated steatotic liver disease: Lastly, as in other experimental models of NAFLD (Figures 1 and 2), Tn housing did not modify hepatic expression of insulin signaling (Pparγ and Irs1) or lipid metabolism-associated genes (Lxrα, Lipe, Chrebp, and Srebp) (Supplementary Figures 7M, N).