RNA-seq analysis of FTO KD revealed the distinct underlying molecular mechanisms and signaling pathways associated with antitumor activities in colorectal cancer cells through a set of differentially expressed genes including the top 4 down-regulated genes (EREG, KRAP, PDE4B and SLC38A2) confirmed by qPCR (Figures 6D, E). The gene discussed is ITPRID2; the disease is colorectal cancer.