Then, the KEGG method was used to assess the signaling mechanisms using the CRG-DEGs, which of the top 10 pathways were shown in Figure 2G, including Glycolysis/Gluconeogenesis, Cell cycle, Vascular smooth muscle contraction, Amino sugar and nucleotide sugar metabolism, Retinol metabolism, Central carbon metabolism in cancer, p53 signaling pathway, Apoptosis, Metabolic pathways, and Fc gamma R-mediated phagocytosis. The gene discussed is CHD7; the disease is cancer.