PNPLA2 and steatosis: In our model, IH reinforced the expression of lipogenesis-associated factors, Chrebp and Fasn, and decreased ATGL expression, resulting in the exacerbated eWAT steatosis and diminished lipolysis in HFHFD eWAT (Figure 5), which also explained the reduced serum FFA level in the HFHFD + IH.