Immunofluorescence assays for MPO and cit-H3 demonstrate higher presence of NETs in grade IV gliomas than in grade II and III tumors. NETs activate RAGE receptors on glioma cells to enhance proliferation, migration, invasiveness, and IL-8 production in vitro. Tumor-derived IL-8 drives neutrophil recruitment to the tumor and NET production (138). This evidence concerns the gene MPO and central nervous system cancer.