As shown in Fig. 1C–E, analysis revealed that the higher expression levels of TUBB2A, TUBB2B, and TUBB3 were associated with shorter OS (p < 0.05) in the TCGA HCC cohort, while higher mRNA levels of TUBB2B and TUBB3 were associated with shorter OS in the GSE14520 cohort (p < 0.05) (Fig. 1F, G). This evidence concerns the gene TUBB3 and hepatocellular carcinoma.