PRKAA2 and cancer: To better define the epigenetic regulatory role of AMPK in cancer metastasis, we intended to focus on investigating AMPKα2 subunit which prefers nuclear localization than AMPKα1.19 We found that AMPKα2 deficiency enhanced the migration and invasion of the lung cancer cell H1299 in the transwell assay (Supplementary Fig. 1a, b).