Brakenhoff et al. analyzed the biological signals of 197 HPV-negative (nrgative advanced stage HNSCC) patients with advanced HNSCC before and after radiotherapy and chemotherapy, and the analysis results showed that high CD8+ T profile was closely associated with good prognosis[55].Recent studies have shown that TIL density and PD-L1 expression are correlated with the prognosis of HNC patients. The gene discussed is CD8A; the disease is head and neck squamous cell carcinoma.