SPP1 and Alzheimer disease: Hif1α itself is a DAM gene, and many other important DAM genes (e.g., Spp1, Igf1) are also HIF-responsive genes.17,18,51 Several recent reports have also demonstrated upregulated HIF1 signaling in AD and collectively point to concurrent activation of both Hif1α and OxPhos gene expression as a common feature of amyloid-responding microglia in both humans and mice.37,52-54 In line with this, our data also demonstrate that Hif1α was a predicted TF enriched in Mi_6.