Decreased expression of one of these genes, Lgals3 (galectin-3), was recently found to protect against retinal ganglion cell (RGC) loss, with E4 microglia failing to upregulate Lgals3 and assume an MgND profile in response to the increased intraocular pressure, a model of glaucoma.50 In the current study, we observed increased microglial expression of Lgals3 in aged E4 microglia, whereas young E4 microglia had decreased expression relative to E3. This evidence concerns the gene LGALS3 and glaucoma.