KRAS and lung cancer: To obtain a comprehensive view of the immune atlas for LKB1 deficient lung cancer, we utilized CRISPR/Cas9-mediated gene knockout in the genetically engineered Kras-driven mouse model to generate KrasG12D/+ mice with conditional knockout of Lkb1. These animals were treated with nasal inhalation of lentiviruses targeting Lkb1 (KL) or Tomato (K, referred to as negative control) (Fig. 1a).