To obtain a comprehensive view of the immune atlas for LKB1 deficient lung cancer, we utilized CRISPR/Cas9-mediated gene knockout in the genetically engineered Kras-driven mouse model to generate KrasG12D/+ mice with conditional knockout of Lkb1. These animals were treated with nasal inhalation of lentiviruses targeting Lkb1 (KL) or Tomato (K, referred to as negative control) (Fig. 1a). Here, KRAS is linked to lung carcinoma.