To address the question how SPI2-T3SS effector proteins reach their subcellular destination in an infected host cell, we applied LCI by confocal laser-scanning microscopy (CLSM) of infected HeLa LAMP1-GFP cells at 4 h p.i. In the early stage of infection, the SCV is already formed, while SIF biogenesis initiates. The gene discussed is LAMP1; the disease is infection.