Biochemical analysis of key markers reflecting potential systemic imbalances including LDH, Creatinine, Urea, AST (ASAT), ALT (ALAT), Potassium, CPK as well as the malnutrition marker albumin, did not reveal significant differences between the different groups suggesting that the dietary approach was well-tolerated (Additional file 6: Fig. S6c). Here, GPT is linked to nutritional deficiency disease.