Biochemical analysis of key markers reflecting potential systemic imbalances including LDH, Creatinine, Urea, AST (ASAT), ALT (ALAT), Potassium, CPK as well as the malnutrition marker albumin, did not reveal significant differences between the different groups suggesting that the dietary approach was well-tolerated (Additional file 6: Fig. S6c). This evidence concerns the gene PIK3C2A and nutritional deficiency disease.