Treatment of A549 (lung cancer), T47D (breast cancer) and PC3 (prostate cancer) cells with Rapamycin or Torin 1 similarly suppressed the protein and mRNA expression of B7-H3 (Supplementary Fig. 1a–f), further validating that mTORC1 is a critical regulator of B7-H3 expression. This evidence concerns the gene CD276 and prostate cancer.