In addition, we did not observe a difference when comparing pSTAT1 and STAT1 levels in B7-H3 proficient and deficient cells in the presence of anti-CD3/CD28 beads-activated T cells isolated from the spleen of naïve mice (Supplementary Fig. 5d), implying that the tumor microenvironment is required for the enhanced IFN-γ responses in tumor cells with B7-H3 inhibition. This evidence concerns the gene CD28 and neoplasm.