In this experiment, it was confirmed that miR-136 is negatively regulated by PVT1, which can inhibit the cell proliferation, migration, and invasion of endometrial cancer non-stem cells and ECSCs; enhance cell apoptosis; block the cell cycle; reduce stem cell self-renewal, chemotherapy, and TSC characteristics, such as drug resistance and stem cell marker expression; and restore the effect of PVT1 on endometrial cancer non-stem cells and ECSCs. Here, PVT1 is linked to endometrial cancer.