MAPK8 and neuroblastoma: To investigate whether the heterogeneity in JNK activation predicted by our patient-specific simulations also translated to the emergence of single-cell heterogeneity, we used five-color flow cytometry to measure the relative single-cell expression of each model component in the SH-SY5Y neuroblastoma cell line, a thrice subcloned derivative of the SK-N-SH line (16).