TP53 and neoplasm: Of note was the near triploidy case (PD37195), with a germ line TP53 (p.N239D, 17:7577566 T>C; variant allele frequency [VAF] in healthy tissue = 0.29; tumor VAF = 0.87 with chr17 copy neutral loss of heterozygosity [LOH]) mutation (supplemental Table 8).