By interaction relationship analysis of the DEGs of Resinacein S regulated in human liver cells and the target genes of Resinacein S against NAFLD from public database, we found 8 proteins TNF, PIK3CA, AKT1, AKT2, ESR1, CYP3A4, CYP17A1, and PTPN11 may be the hub proteins of Resinacein S regulated against NAFLD. Here, ESR1 is linked to metabolic dysfunction-associated steatotic liver disease.