To address the role of TNFα in a genetic model of hepatic steatosis, TNFR1−/− mice (TKO) were crossed with mice lacking peroxisome proliferator activated receptor alpha (PKO) to generate double mutant PPARα−/− TNFR1−/− mice (PTKO). Here, TNFRSF1A is linked to Hepatic steatosis.