TNFR2 expression by CD4 T and CD8 T cell metaclusters in other 17 human cancer types were analyzed as well, and results consistently showed that TNFR2-expressing cells are mostly concentrated by Tregs in CD4 T cells, while distribution of TNFR2-expressing cells are less homogenous in the CD8 T cells in these cancer types (Supplementary Figure 1). This evidence concerns the gene CD4 and cancer.