To verify the relationship of TIM-3, TNF-α, or IFN-γ protein expression with the clinicopathologic parameters of LAUD, gender, age, TNM stages, lymph node metastasis, smoke, CEA, BMI, location, tumor size, and date of operation were investigated in this study (Tables 1, –3). The gene discussed is TNF; the disease is metastatic malignant neoplasm in the lymph nodes.