To identify stem-like phenotypes in our tumor nuclei population, we investigated the expression levels of classically used markers of cancer stem cells (ITGA6, CD44, PROM1, NES, MSI1, MYC, NANOG, SOX1, SOX2, POU5F1, VIM, SDC1, SDC2, GPC1, GPC2), as well as an enrichment score for stemness from Tirosh et al29,30. This evidence concerns the gene NES and neoplasm.